Calypso®
Label-free, immobilization-free characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.
CG-MALS
The Calypso® II, in conjunction with a DAWN® or miniDAWN® MALS detector, measures binding affinities and absolute, molecular stoichiometries of complex biomolecular interactions.
Weight-Average Molar Mass
In addition to equilibrium association constants Kd and absolute stoichiometry of reversible self- and hetero-associations, it can also help determine reaction or aggregation rates, non-specific interaction parameters (virial coefficients) and even automate measurements of weight-average molar mass and dn/dc for polymers.
CALYPSO Software
Included with each Calypso is a copy of CALYPSO™ software, the most versatile software package available for analysis of biomolecular interactions in solution by light scattering.
Creating composition gradients...
CG-MALS employs a Calypso connected to a MALS instrument and optional concentration detector. The Calypso performs sample preparation and delivery, combining up to three different solution in precise mixing ratios and injecting into the detectors.
Accurate compositions result from setting the Calypso's pumps to appropriate flow rate ratios, all controlled from the CALYPSO software through an intuitive GUI. Mixing is achieved by dispensing the solutions simultaneously from all three pumps via a static mixer.
...that work with MALS detectors
Reliable light scattering measurement require the samples to be degassed and filtered. The Calypso incorporates built-in, low-volume degassers and filter housings, connected via PEEK tubing and finger-tight fittings. Even the filter housing is finger-tight, making use of a proprietary design for maximum convenience.
Calypso features
- Biocompatible wetted materials.
- Flexibility to re-organize fluid paths and utilize different wetted materials as needed.
- Convenient loading of sample and diluent solutions via conical tubes: 5, 15 or 50 mL.
- Autoinject Out contact closure signal, to trigger data acquisition by ASTRA® or DYNAMICS® software for data acquisition and analyses not supported by the CALYPSO software (e.g., DLS acquisition).
- Built-in Wash port, allowing the pumps to draw on reservoirs of wash solutions for post-experimental clean-up. Automation of up to two sequential wash solutions, e.g. buffer (to clean out proteins) followed by water (to clean out buffer salts) or detergent followed by water, is possible when an Orbit™ solvent selector valve is added to the setup. These operations are appended to the Method for unattended operation.