Application Note

Performance Stability Of CORTECS 2.7 μm Columns Following Repeated Injections Of Precipitated Plasma

Source: Waters Corporation

By Jonathan P. Danaceau, Erin E. Chambers, Bonnie Alden, and Kenneth J. Fountain, Waters Corporation, Milford, MA USA

Bioprocess Bioreactor GettyImages-532549138

Maintaining LC column performance is often a consideration when working with biological samples. In general, simple sample preparation techniques such as dilution or protein precipitation can result in reduced column lifetimes when compared to more selective sample cleanup procedures (e.g., solid-phase extraction [SPE]). CORTECS 2.7 μm Columns, made using optimally packed solid-core particles, are designed to be highly efficient, scalable, and robust. This includes the ability to maintain excellent chromatographic performance even after repeated exposure to minimally processed biological samples. This technical note details the ruggedness of a chromatographic separation of a panel of synthetic cannabinoid drugs and metabolites following repeated injections of precipitated plasma. These compounds represent a growing challenge for forensic and law enforcement laboratories. Due to the fact that new variants of these compounds are constantly being introduced, laboratories will often rely on relatively non-selective sample preparation techniques that ensure the efficient recovery of a wide variety metabolites and chemotypes. As a result, chromatographic columns need to be able to tolerate repeated injections of minimally processed samples, while maintaining assay performance.

access the Application Note!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Clinical Leader? Subscribe today.

Subscribe to Clinical Leader X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Clinical Leader