Product/Service

Protein Analysis

Source: SGS Life Sciences Services
SGS's Life Science Services has the necessary expertise and experience to perform the analysis of your proteins, peptides or antibodies.

SGS's Life Science Services has the necessary expertise and experience to perform the analysis of your proteins, peptides or antibodies. We offer a broad portfolio of analytical test methods ranging from classical SDS-PAGE and blotting techniques, various chromatographic methods to the latest mass spectrometry based detection techniques.

The experts at SGS will help you to check for purity and homogeneity of your protein, identify possible impurities, analyze glycosylation patterns, detect aggregation complexes (especially important in antibody formulations), and test for the biological activity.

Protein Analysis Technique:

Protein Purity

  • RP-HPLC (UV, ELSD, fluorescence)
  • IE-HPLC
  • SEC-HPLC
  • SDS-PAGE (reduced, non-reduced)
  • IEF
  • N-terminal sequencing
  • Variants analysis (LC/MS/MS)
  • Degradation products (LC/MS/MS)

Protein Identity

  • IEF
  • Western Blotting
  • Peptide mapping and intact protein mass analysis
  • Amino Acid Analysis
  • Disulfide bridges characterization
  • Exact mass determination
  • Sequence confirmation (LC/MS/MS)
  • Phosphorylation status
  • Methionine oxidation status
  • Antibody typing and subclasses analysis
  • PEGylation status

Protein/Antibody Aggregation Analysis

  • HPLC-SEC/ MALLS

Molecular Mass Analysis

  • LC/MS/MS (QTof)
  • HPLC/MALLS
  • SDS-PAGE

    • Glycosylation Analysis

    • Carbohydrate composition analysis (HPAEC-PAD)
    • Glycosylation sites (LC/MS/MS)
    • Glycan chains integrity

    Biological Activity

    • ELISA
    • Enzymatic assays
    • Bioassays
    • Protein concentration
    • Confirmation of Immunogenicity (rabbits, rats, mice or guinea pigs)
    • Production of polyclonal antibodies
    • Challenge test

    Sterility

    • Pyrogenicty/endotoxins testing
    • pH
    • Appearance